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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411
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        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        Report generated on 2025-02-24, 11:31 CET based on data in: /home/benlloch/parkinson/analysis/fastqs/fastqc_reports

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        General Statistics

        Showing 168/168 rows and 3/6 columns.
        Sample NameDupsGCAvg lenMedian lenFailedSeqs
        SRR22002247_1
        58.7%
        50.0%
        150bp
        150bp
        27%
        56.1M
        SRR22002247_2
        57.8%
        51.0%
        150bp
        150bp
        18%
        56.1M
        SRR22002248_1
        60.1%
        51.0%
        150bp
        150bp
        27%
        51.8M
        SRR22002248_2
        59.8%
        51.0%
        150bp
        150bp
        18%
        51.8M
        SRR22002249_1
        59.4%
        50.0%
        150bp
        150bp
        27%
        52.5M
        SRR22002249_2
        59.0%
        50.0%
        150bp
        150bp
        18%
        52.5M
        SRR22002250_1
        52.5%
        49.0%
        150bp
        150bp
        27%
        54.7M
        SRR22002250_2
        51.9%
        50.0%
        150bp
        150bp
        18%
        54.7M
        SRR22002251_1
        62.0%
        53.0%
        150bp
        150bp
        27%
        54.0M
        SRR22002251_2
        61.9%
        53.0%
        150bp
        150bp
        18%
        54.0M
        SRR22002252_1
        49.7%
        48.0%
        150bp
        150bp
        9%
        51.9M
        SRR22002252_2
        48.2%
        49.0%
        150bp
        150bp
        0%
        51.9M
        SRR22002253_1
        66.7%
        51.0%
        150bp
        150bp
        36%
        52.4M
        SRR22002253_2
        66.1%
        52.0%
        150bp
        150bp
        18%
        52.4M
        SRR22002254_1
        58.9%
        49.0%
        150bp
        150bp
        27%
        50.3M
        SRR22002254_2
        58.5%
        50.0%
        150bp
        150bp
        18%
        50.3M
        SRR22002255_1
        51.0%
        49.0%
        150bp
        150bp
        27%
        52.5M
        SRR22002255_2
        50.0%
        49.0%
        150bp
        150bp
        18%
        52.5M
        SRR22002256_1
        61.2%
        50.0%
        150bp
        150bp
        27%
        54.7M
        SRR22002256_2
        60.6%
        50.0%
        150bp
        150bp
        18%
        54.7M
        SRR22002257_1
        61.8%
        50.0%
        150bp
        150bp
        27%
        52.2M
        SRR22002257_2
        61.5%
        50.0%
        150bp
        150bp
        18%
        52.2M
        SRR22002258_1
        52.6%
        49.0%
        150bp
        150bp
        27%
        52.2M
        SRR22002258_2
        51.7%
        49.0%
        150bp
        150bp
        18%
        52.2M
        SRR22002259_1
        56.3%
        49.0%
        150bp
        150bp
        27%
        52.4M
        SRR22002259_2
        55.3%
        49.0%
        150bp
        150bp
        18%
        52.4M
        SRR22002260_1
        59.7%
        50.0%
        150bp
        150bp
        27%
        53.4M
        SRR22002260_2
        59.5%
        51.0%
        150bp
        150bp
        18%
        53.4M
        SRR22002261_1
        46.4%
        48.0%
        150bp
        150bp
        9%
        52.7M
        SRR22002261_2
        45.2%
        48.0%
        150bp
        150bp
        9%
        52.7M
        SRR22002262_1
        61.2%
        50.0%
        150bp
        150bp
        27%
        52.6M
        SRR22002262_2
        60.8%
        50.0%
        150bp
        150bp
        18%
        52.6M
        SRR22002263_1
        59.0%
        49.0%
        150bp
        150bp
        27%
        52.3M
        SRR22002263_2
        57.5%
        50.0%
        150bp
        150bp
        18%
        52.3M
        SRR22002264_1
        61.9%
        51.0%
        150bp
        150bp
        27%
        52.3M
        SRR22002264_2
        61.4%
        51.0%
        150bp
        150bp
        18%
        52.3M
        SRR22002265_1
        47.5%
        49.0%
        150bp
        150bp
        9%
        54.1M
        SRR22002265_2
        45.9%
        49.0%
        150bp
        150bp
        0%
        54.1M
        SRR22002266_1
        62.3%
        50.0%
        150bp
        150bp
        27%
        57.2M
        SRR22002266_2
        62.4%
        50.0%
        150bp
        150bp
        18%
        57.2M
        SRR22002267_1
        60.7%
        49.0%
        150bp
        150bp
        27%
        58.2M
        SRR22002267_2
        60.4%
        50.0%
        150bp
        150bp
        18%
        58.2M
        SRR22002268_1
        62.6%
        50.0%
        150bp
        150bp
        27%
        62.4M
        SRR22002268_2
        62.4%
        50.0%
        150bp
        150bp
        18%
        62.4M
        SRR22002269_1
        50.5%
        49.0%
        150bp
        150bp
        27%
        56.0M
        SRR22002269_2
        49.4%
        49.0%
        150bp
        150bp
        9%
        56.0M
        SRR22002270_1
        52.4%
        49.0%
        150bp
        150bp
        27%
        50.2M
        SRR22002270_2
        50.8%
        50.0%
        150bp
        150bp
        18%
        50.2M
        SRR22002271_1
        58.4%
        51.0%
        150bp
        150bp
        27%
        52.2M
        SRR22002271_2
        58.1%
        51.0%
        150bp
        150bp
        18%
        52.2M
        SRR22002272_1
        55.6%
        49.0%
        150bp
        150bp
        27%
        65.9M
        SRR22002272_2
        54.7%
        49.0%
        150bp
        150bp
        18%
        65.9M
        SRR22002273_1
        60.9%
        49.0%
        150bp
        150bp
        27%
        65.5M
        SRR22002273_2
        60.8%
        50.0%
        150bp
        150bp
        18%
        65.5M
        SRR22002274_1
        68.5%
        50.0%
        150bp
        150bp
        27%
        71.7M
        SRR22002274_2
        68.4%
        50.0%
        150bp
        150bp
        18%
        71.7M
        SRR22002275_1
        60.0%
        49.0%
        150bp
        150bp
        27%
        63.9M
        SRR22002275_2
        59.6%
        50.0%
        150bp
        150bp
        18%
        63.9M
        SRR22002276_1
        60.6%
        49.0%
        150bp
        150bp
        27%
        68.8M
        SRR22002276_2
        60.6%
        50.0%
        150bp
        150bp
        18%
        68.8M
        SRR22002277_1
        59.8%
        49.0%
        150bp
        150bp
        27%
        67.4M
        SRR22002277_2
        59.4%
        49.0%
        150bp
        150bp
        18%
        67.4M
        SRR22002278_1
        61.4%
        49.0%
        150bp
        150bp
        27%
        61.9M
        SRR22002278_2
        60.8%
        49.0%
        150bp
        150bp
        18%
        61.9M
        SRR22002279_1
        51.0%
        48.0%
        150bp
        150bp
        18%
        68.1M
        SRR22002279_2
        49.9%
        48.0%
        150bp
        150bp
        0%
        68.1M
        SRR22002280_1
        66.1%
        50.0%
        150bp
        150bp
        27%
        64.1M
        SRR22002280_2
        65.0%
        50.0%
        150bp
        150bp
        18%
        64.1M
        SRR22002281_1
        60.6%
        50.0%
        150bp
        150bp
        27%
        54.2M
        SRR22002281_2
        60.1%
        50.0%
        150bp
        150bp
        18%
        54.2M
        SRR22002282_1
        53.6%
        47.0%
        150bp
        150bp
        18%
        60.1M
        SRR22002282_2
        53.0%
        47.0%
        150bp
        150bp
        9%
        60.1M
        SRR22002283_1
        53.8%
        48.0%
        150bp
        150bp
        27%
        67.0M
        SRR22002283_2
        52.8%
        49.0%
        150bp
        150bp
        18%
        67.0M
        SRR22002284_1
        64.4%
        49.0%
        150bp
        150bp
        27%
        67.0M
        SRR22002284_2
        63.6%
        50.0%
        150bp
        150bp
        18%
        67.0M
        SRR22002285_1
        63.0%
        49.0%
        150bp
        150bp
        27%
        60.9M
        SRR22002285_2
        62.1%
        50.0%
        150bp
        150bp
        18%
        60.9M
        SRR22002286_1
        64.3%
        51.0%
        150bp
        150bp
        36%
        60.6M
        SRR22002286_2
        64.2%
        51.0%
        150bp
        150bp
        18%
        60.6M
        SRR22002287_1
        61.3%
        49.0%
        150bp
        150bp
        27%
        61.9M
        SRR22002287_2
        60.9%
        50.0%
        150bp
        150bp
        18%
        61.9M
        SRR22002288_1
        62.7%
        49.0%
        150bp
        150bp
        27%
        65.0M
        SRR22002288_2
        62.3%
        50.0%
        150bp
        150bp
        18%
        65.0M
        SRR22002289_1
        51.7%
        48.0%
        150bp
        150bp
        18%
        55.6M
        SRR22002289_2
        50.0%
        48.0%
        150bp
        150bp
        9%
        55.6M
        SRR22002290_1
        62.0%
        49.0%
        150bp
        150bp
        27%
        69.2M
        SRR22002290_2
        61.3%
        49.0%
        150bp
        150bp
        18%
        69.2M
        SRR22002291_1
        59.4%
        49.0%
        150bp
        150bp
        27%
        65.1M
        SRR22002291_2
        57.4%
        49.0%
        150bp
        150bp
        18%
        65.1M
        SRR22002292_1
        58.1%
        50.0%
        150bp
        150bp
        27%
        57.3M
        SRR22002292_2
        56.8%
        50.0%
        150bp
        150bp
        18%
        57.3M
        SRR22002293_1
        57.4%
        48.0%
        150bp
        150bp
        27%
        70.0M
        SRR22002293_2
        55.8%
        48.0%
        150bp
        150bp
        18%
        70.0M
        SRR22002294_1
        60.2%
        49.0%
        150bp
        150bp
        27%
        62.9M
        SRR22002294_2
        60.1%
        49.0%
        150bp
        150bp
        18%
        62.9M
        SRR22002295_1
        60.2%
        51.0%
        150bp
        150bp
        9%
        66.5M
        SRR22002295_2
        59.2%
        51.0%
        150bp
        150bp
        18%
        66.5M
        SRR22002296_1
        46.5%
        46.0%
        150bp
        150bp
        0%
        78.4M
        SRR22002296_2
        45.7%
        46.0%
        150bp
        150bp
        0%
        78.4M
        SRR22002297_1
        55.7%
        49.0%
        150bp
        150bp
        27%
        73.4M
        SRR22002297_2
        55.1%
        49.0%
        150bp
        150bp
        18%
        73.4M
        SRR22002298_1
        61.1%
        49.0%
        150bp
        150bp
        27%
        61.7M
        SRR22002298_2
        60.8%
        49.0%
        150bp
        150bp
        18%
        61.7M
        SRR22002299_1
        60.7%
        49.0%
        150bp
        150bp
        27%
        61.3M
        SRR22002299_2
        60.2%
        49.0%
        150bp
        150bp
        18%
        61.3M
        SRR22002300_1
        46.3%
        48.0%
        150bp
        150bp
        18%
        53.7M
        SRR22002300_2
        44.9%
        48.0%
        150bp
        150bp
        9%
        53.7M
        SRR22002301_1
        39.8%
        48.0%
        150bp
        150bp
        0%
        60.8M
        SRR22002301_2
        38.0%
        47.0%
        150bp
        150bp
        9%
        60.8M
        SRR22002302_1
        49.4%
        48.0%
        150bp
        150bp
        0%
        60.7M
        SRR22002302_2
        47.7%
        48.0%
        150bp
        150bp
        9%
        60.7M
        SRR22002303_1
        63.8%
        49.0%
        150bp
        150bp
        27%
        68.8M
        SRR22002303_2
        64.0%
        49.0%
        150bp
        150bp
        18%
        68.8M
        SRR22002304_1
        48.8%
        48.0%
        150bp
        150bp
        18%
        64.5M
        SRR22002304_2
        48.0%
        48.0%
        150bp
        150bp
        9%
        64.5M
        SRR22002305_1
        54.9%
        49.0%
        150bp
        150bp
        18%
        68.7M
        SRR22002305_2
        54.0%
        49.0%
        150bp
        150bp
        18%
        68.7M
        SRR22002306_1
        51.0%
        49.0%
        150bp
        150bp
        27%
        52.8M
        SRR22002306_2
        49.6%
        49.0%
        150bp
        150bp
        9%
        52.8M
        SRR22002307_1
        50.1%
        49.0%
        150bp
        150bp
        18%
        54.9M
        SRR22002307_2
        49.0%
        49.0%
        150bp
        150bp
        9%
        54.9M
        SRR22002308_1
        47.6%
        47.0%
        150bp
        150bp
        9%
        61.7M
        SRR22002308_2
        46.3%
        47.0%
        150bp
        150bp
        9%
        61.7M
        SRR22002309_1
        55.0%
        49.0%
        150bp
        150bp
        27%
        57.6M
        SRR22002309_2
        54.2%
        49.0%
        150bp
        150bp
        18%
        57.6M
        SRR22002310_1
        61.4%
        49.0%
        150bp
        150bp
        27%
        74.5M
        SRR22002310_2
        60.0%
        49.0%
        150bp
        150bp
        18%
        74.5M
        SRR22002311_1
        58.1%
        50.0%
        150bp
        150bp
        18%
        54.5M
        SRR22002311_2
        56.9%
        50.0%
        150bp
        150bp
        18%
        54.5M
        SRR22002312_1
        50.1%
        49.0%
        150bp
        150bp
        18%
        63.7M
        SRR22002312_2
        48.5%
        49.0%
        150bp
        150bp
        9%
        63.7M
        SRR22002313_1
        60.4%
        49.0%
        150bp
        150bp
        18%
        68.3M
        SRR22002313_2
        59.4%
        49.0%
        150bp
        150bp
        18%
        68.3M
        SRR22002314_1
        58.7%
        49.0%
        150bp
        150bp
        18%
        64.9M
        SRR22002314_2
        57.8%
        49.0%
        150bp
        150bp
        18%
        64.9M
        SRR22002315_1
        53.3%
        49.0%
        150bp
        150bp
        18%
        54.1M
        SRR22002315_2
        52.5%
        49.0%
        150bp
        150bp
        18%
        54.1M
        SRR22002316_1
        54.6%
        50.0%
        150bp
        150bp
        18%
        54.6M
        SRR22002316_2
        53.5%
        50.0%
        150bp
        150bp
        18%
        54.6M
        SRR22002317_1
        53.2%
        47.0%
        150bp
        150bp
        20%
        59.0M
        SRR22002317_2
        49.2%
        48.0%
        150bp
        150bp
        0%
        59.0M
        SRR22002318_1
        55.4%
        47.0%
        150bp
        150bp
        20%
        74.4M
        SRR22002318_2
        51.5%
        48.0%
        150bp
        150bp
        10%
        74.4M
        SRR22002319_1
        61.0%
        48.0%
        150bp
        150bp
        18%
        68.8M
        SRR22002319_2
        57.6%
        48.0%
        150bp
        150bp
        9%
        68.8M
        SRR22002320_1
        61.9%
        48.0%
        150bp
        150bp
        18%
        75.1M
        SRR22002320_2
        59.4%
        48.0%
        150bp
        150bp
        18%
        75.1M
        SRR22002321_1
        60.6%
        48.0%
        150bp
        150bp
        18%
        72.3M
        SRR22002321_2
        57.4%
        48.0%
        150bp
        150bp
        9%
        72.3M
        SRR22002322_1
        52.8%
        47.0%
        150bp
        150bp
        20%
        68.3M
        SRR22002322_2
        49.6%
        47.0%
        150bp
        150bp
        0%
        68.3M
        SRR22002323_1
        62.1%
        47.0%
        150bp
        150bp
        18%
        93.5M
        SRR22002323_2
        57.9%
        48.0%
        150bp
        150bp
        9%
        93.5M
        SRR22002324_1
        62.3%
        48.0%
        150bp
        150bp
        18%
        85.7M
        SRR22002324_2
        59.0%
        48.0%
        150bp
        150bp
        9%
        85.7M
        SRR22002325_1
        66.7%
        48.0%
        150bp
        150bp
        18%
        79.2M
        SRR22002325_2
        63.8%
        49.0%
        150bp
        150bp
        9%
        79.2M
        SRR22002326_1
        58.5%
        48.0%
        150bp
        150bp
        18%
        72.0M
        SRR22002326_2
        54.9%
        48.0%
        150bp
        150bp
        9%
        72.0M
        SRR22002327_1
        58.3%
        48.0%
        150bp
        150bp
        18%
        71.2M
        SRR22002327_2
        55.2%
        48.0%
        150bp
        150bp
        9%
        71.2M
        SRR22002328_1
        64.4%
        48.0%
        150bp
        150bp
        18%
        73.0M
        SRR22002328_2
        60.7%
        48.0%
        150bp
        150bp
        9%
        73.0M
        SRR22002329_1
        63.3%
        48.0%
        150bp
        150bp
        18%
        72.4M
        SRR22002329_2
        59.8%
        49.0%
        150bp
        150bp
        9%
        72.4M
        SRR22002330_1
        66.0%
        49.0%
        150bp
        150bp
        18%
        79.5M
        SRR22002330_2
        63.2%
        49.0%
        150bp
        150bp
        9%
        79.5M

        FastQC

        Version: 0.12.1

        Quality control tool for high throughput sequencing data.URL: http://www.bioinformatics.babraham.ac.uk/projects/fastqc

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Created with MultiQC

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Created with MultiQC

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Created with MultiQC

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Created with MultiQC

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Created with MultiQC

        Sequence Length Distribution

        All samples have sequences of a single length (150bp)

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (e.g. PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Created with MultiQC

        Overrepresented sequences by sample

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Created with MultiQC

        Top overrepresented sequences

        Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

        Showing 20/20 rows and 3/3 columns.
        Overrepresented sequenceReportsOccurrences% of all reads
        CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC
        88
        27445727
        0.2619%
        GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT
        84
        16246599
        0.1550%
        CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT
        83
        17380682
        0.1659%
        CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT
        82
        10830698
        0.1033%
        CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT
        82
        10592608
        0.1011%
        CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC
        81
        9656073
        0.0921%
        CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA
        77
        12831644
        0.1224%
        CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGG
        74
        7885764
        0.0752%
        CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG
        73
        9898581
        0.0945%
        CGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGG
        72
        7385095
        0.0705%
        CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC
        69
        8278804
        0.0790%
        GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA
        66
        5815467
        0.0555%
        GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC
        65
        6829216
        0.0652%
        GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGTGGGAGGA
        62
        5334006
        0.0509%
        CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC
        58
        5613237
        0.0536%
        GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT
        57
        6400407
        0.0611%
        GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA
        56
        6197475
        0.0591%
        CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT
        52
        4265736
        0.0407%
        CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT
        50
        4517339
        0.0431%
        GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT
        49
        5094470
        0.0486%

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Created with MultiQC

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        Created with MultiQC

        Software Versions

        Software Versions lists versions of software tools extracted from file contents.

        SoftwareVersion
        FastQC0.12.1